甲胺磷对鸡坐骨神经中微管和微丝蛋白水平的影响

Effects of methamidophos on microtubule and microfilament protiens in sciatic nerve of hens

目的 研究甲胺磷诱发迟发性神经病动物的坐骨神经中微管蛋白和微丝中β肌动蛋白水平随时间变化的趋势。方法 母鸡以30mg/kg甲胺磷连续15d经皮下注射染毒,出现迟发性神经病症状后的第2、10和2 3天,分别取其坐骨神经匀浆,用Westernblotting法检测鸡坐骨神经中α微管蛋白、β微管蛋白和β肌动蛋白的水平。结果 鸡坐骨神经上清液中α微管蛋白水平随时间延长逐渐下降,出现迟发性神经病症状后的第2、10和2 3天分别降低了6 %、15 %和2 5 % ,沉淀中变化不明显;β微管蛋白水平在各时间点均降低:在第2、10和2 3天,沉淀中的水平分别下降了2 7%、6 %和19% ,上清液则分别降低了1%、2 1%和2 2 % ;沉淀中β肌动蛋白水平升高:第2、10和2 3天分别升高了2 4 %、4 8%和17% ;上清液中β肌动蛋白水平的变化不明显。结论 甲胺磷可导致母鸡坐骨神经α微管蛋白、β微管蛋白和β肌动蛋白改变,可能是甲胺磷诱发动物迟发性神经病的机制之一。

Objective To investigate the dynamic changes of α-tubulin,β-tubulin and β-actin in sciatic nerve of hen with organophosphorus ester-induced delayed neuropathy (OPIDN). Methods OPIDN was induced in 10-month-old Roman hens by daily subcutaneous administration of 30 mg/kg methamidophos for 15 days.Hens were sacrificed 2,10,and 23 days respectively after manifesting neuropathy.The sciatic nerves were dissected,homogenized and used for the determination of the α-tubulin ,β-tubulin and β-actin levels by western blotting. Results The levels of α-tubulin in supernatant of sciatic nerves were decreased by 6%,15%and 25% respectively on day 2,10 and 23 respectively,while those in pellet remained almost unchanged.β-tubulin were decreased by 27%,6%,19% in pellet and 1%,21%,22% in supernatant of sciatic nerves on 2,10 and 23 days.β-actin level in pellet of sciatic nerve increased by 24%,48% and 17% on day 2,10 and 23,and little changes were observed in supernatant. Conclusion Methamidophos may induced changes of α-tubulin,β-tubulin and β-actin levels in sciatic nerve of hen,which may be one of the mechanism of the contribution to the occurrence and development of OPIDN.