摘要
目的:观测针刺对STZ所导致的糖尿病大鼠下丘脑NPY及其mRNA表达的影响。方法:Wistar大鼠4 3只,1 3只为正常对照,另30只行链脲左菌素腹腔注射造模,造模成功的大鼠随机分为模型组和针刺治疗组(针刺“胰俞”“足三里”“关元”) ,针刺治疗2个疗程后取脑,运用免疫组化法和原位杂交法分别检测下丘脑NPY蛋白及其mRNA的表达。结果:STZ糖尿病大鼠下丘脑室旁核、弓状核NPY阳性纤维较正常组大鼠明显增多,下丘脑外侧区NPYmRNA表达较正常明显增多(P <0 .0 5 ) ,针刺治疗后糖尿病大鼠下丘脑室旁核、弓状核NPY阳性纤维及下丘脑外侧区NPYmRNA表达明显减少(P <0 .0 5 )。结论:针刺可以降低STZ糖尿病大鼠下丘脑增多的NPY的合成及其含量,这可能是针刺改善糖尿病能量代谢的中枢机制之一。
Objective: To observe the effect of electroacupuncture (EA) on the expression of NPY and its mRNA in hypothalamus of streptozotocin (STZ)-induced diabetes mellitus (DM) rats. Methods: Forty-three Wistar rats (180～220 g) were assigned to normal control group (n=13), model group (n=11) and EA group (n=11). DM model was established by intraperitoneal injection of STZ(50 mg/kg). EA (100 Hz, 0.5～2 mA, 15 min) was applied to bilateral “Yishu”,“Zusanli”(ST 36) and “Guanyuan”(CV 4), once daily and with 6 sessions being a therapeutic course. After two courses of treatment, the rats were sacrificed to test the expression of NPY and its mRNA in hypothalamus with immunohistochemical and in situ hybridization methods respectively. Results: Compared with normal group (n=5), OD values of NPY expression positive fibers in hypothalamic paraventricular nucleus (PVN) and arcuate nucleus (ARC), the OD value and the total area of NPYmRNA expression in lateral hypothalamus (LH) increased significantly (P<0.05) in DM rats of model group (n=5). Comparison between model group and EA group (n=5) showed that OD values of NPY expression positive fibers in PVN and ARC, the OD value and the total area of NPYmRNA expression in LH of the later group were significantly lower than those of the former group (P<0.05), displaying that after EA, the concentration of NPY and expression of its mRNA in hypothalamus decreased obviously. Conclusion: EA can reduce the level of NPY and its synthesis in hypothalamus of STZ-induced diabetic rats, which may contribute to its effect for improving energy metabolism of diabetes.
出处
《针刺研究》
CAS
CSCD
2005年第1期18-21,共4页
Acupuncture Research
