不同时辰电针对大鼠脊神经节和脊髓内生长抑素mRNA阳性神经元表达的影响

Effect of Electroacupuncture at Different Time on SOMmRNA Expression in the Spinal Ganglia and Spinal Cord in Rats

目的:观察不同时辰电针对大鼠脊神经节和脊髓内生长抑素(SOM )mRNA阳性神经元表达的影响。方法:将2 0只SD大鼠随机分为电针组和对照组,每组又各分为4个时段。采用原位杂交组织化学方法,观察5时、1 1时、1 7时和2 3时2Hz电针大鼠一侧“环跳”穴后,脊神经节和脊髓内SOMmRNA阳性神经元表达。结果:电针大鼠一侧“环跳”穴后,1 1时电针组脊神经节和脊髓后角第Ⅰ、Ⅱ板层SOMmRNA表达增强(P <0 .0 5 )。结论:1 1时2Hz电针对大鼠脊髓和脊神经节内SOMmRNA阳性神经元的表达有上调作用。

Objective:To observe the effects of electroacupuncture (EA) at different time on somatostatin (SOM)mRNA expression in the spinal ganglia and spinal cord in rats. Methods: A total of 40 SD rats were evenly randomized into EA and control groups which were further divided into 23-o'clock (Zi Shi), 5-o'clock (Mao Shi), 11-o'clock (Wu Shi) and 17-o'clock (You Shi) subgroups respectively, with 5 cases being in each subgroup. EA (3 V, 2 Hz, wave width 1 ms) was applied to unilateral “Huantiao”(GB 30) for 30 min. After acupuncture, these rats anesthetized with urethane were infused with 1% paraformaldehyde, 1.25% glutaraldehyde plus 0.1 mol/L HCl buffer solution. Then, L_5 segment of the spinal cord and dorsal root ganglia were taken out to be cut into sections (25 μm). SOMmRNA expression was detected by using in situ hybridization method. Results: After EA of “Huantiao”(GB 30), the number of the densely-stained SOMmRNA positive neurons in the spinal ganglia and laminaⅠandⅡ of the posterior horn of the spinal cord increased markedly at 11-o'clock compared with that of control group at the same time course (P<0.05), but no significant differences were found between two groups at the other time courses. Conclusion: After EA, the expression of SOMmRNA positive neurons in the spinal ganglia and spinal cord is upregulated at 11.00 in rats.