多发性骨髓瘤T细胞亚群及免疫表型分析

Immunophenotype analysis of 32 patients with mutiple myeloma

目的探讨多发性骨髓瘤(MM)患者外周血T细胞亚群变化及骨髓免疫表型特点. 方法用流式细胞仪对本院2000年1月～2004年12月收治的32例MM患者进行外周血T细胞亚群和骨髓免疫表型检测. 结果①初发期和进展期患者外周血CD3、CD4百分率下降,CD4/CD8比值下降,CD8百分率升高.稳定期CD3、CD4及CD8百分率正常,CD4/CD8比值正常.NK细胞在初发期百分率增高,稳定期及进展期与变化不大,正常对照组比较无显著性差异(P>0.05).②初发期患者骨髓免疫进展期表型分型:CD56大部分表达(89.4%),CD54、CD38全部表达,CD19、CD20少量表达(15.8%、5.35%).CD56、CD54和CD38全部表达,CD19、CD20少量表达(20.0%、40%).稳定期CD56少量表达:CD54、CD38全部表达,CD19、CD20不表达.T细胞系标志在初发期少量表达. 结论用流式细胞仪对MM患者外周血T细胞亚群测定及骨髓细胞进行免疫表型分析,有助于MM的诊断及治疗监测.

Objective To explore the changes of T-lymphocy te subsets in peripheral blood and immunophenotype features in bone marrow of the patients with mutiple myeloma(MM). Methods The immuophenotype in bone marrow and the T-lymphocyte subsets in peripheral blood of 32 MM patie nts were detected by flow cytometry (FCM). Results In preth erapy stage and developing stage,the percentages of CD3,CD4 and the ratios CD4/C D8 were decreased, while the percentags of CD8 were increased.The percentages of CD3,CD4, CD8, and the ratio CD4/CD8 in stable stage were normal.To compare wit h normal controls, the percentage of NK cells in pretherapy stage was increased, but there was no obvious changes in stable stage and developing stage.The posit ive rate of CD56,CD54,CD38,CD19 or CD20 in pretherapy stages was 89.4%,100%,100% ,15.8%, and 5.35%,respectively.The positive rate of CD56,CD54,CD38,CD19 or CD2 0 in developing stages was 100%,100%,100%,20%, and 40%,respectively.The positiv e rate of CD56,CD54 or CD38 in stable stage was 37.5 %,100%, and 100%,respec tively, while there were no expression of CD19 and CD20.There were a few express ions of the markes of T-cell in pretherapy stage. Conclusion Immuophenotype analysis in bone marrow and detection of T-lymphocyte subsets in peripheral blood are helpful to the diagosis and monitoring treatment for MM patients.