预热对白血病患者胸腔渗出细胞H_2O_2的影响

Effects of pro-heat on sensitivity of K_(562) cell to hydrogen peroxide

目的探讨预热对肿瘤细胞过氧化氢敏感性的影响及其机制。方法将白血病患者胸腔渗出细胞(K562)在40℃预热不同时间,诱导细胞热应激蛋白70(HSP70)高表达,用RT-PCR检测细胞HSP70mRNA水平;将细胞暴露于0.1,0.2,0.4,0.8mmol的H2O216h,用噻唑蓝(MTT)比色法检测细胞活力,观察预热对不同水平H2O2敏感性的影响;保持H2O2浓度为0.2mmol不变,观察不同预热时间对细胞H2O2敏感性的影响。结果40℃预热后细胞的HSP70含量升高,在预热120min后达到最高,以后缓慢下降,可维持10h以上;预热细胞对不同浓度H2O2的敏感性均显著低于未预热细胞;对于相同浓度的H2O2而言,预热120min的细胞对敏感性最低。结论预热可以引起细胞HSP70表达增高,降低细胞对H2O2的敏感性。

Objective To study the effects of pre-heat on the sensitivity changes of cells to the TNF-α and its mechanism.Methods The K_~562 cells were pre-heated in 40?℃ for different time,then were exposed to the hydrogen peroxide,with different concentrations of 0.1,0.2,0.4,0.8?mmol/L,for 16?hours;at the same time the cell vitalities of different time were detected by MTT with constant exposure to 0.02?mmol/L hydrogen peroxide.Results The HSP70 could be induced by pre-heat,and it went its top after 120?min pre-heat,and the sensitivity of non-heat cell to hydrogen peroxide was lower than that of pre-heated cells.Conclusion Pre-heat could induce the HSP70 expression and inhibit the sensitivity of cell to hydrogen peroxide.The mechanism maybe involved with the over expression of HSP70.