坐骨神经压迫性损伤大鼠背根神经节磷酸化p38MAPK表达的变化

The Changes of Phosphorylated p38MAPK Expression in Dorsal Root Ganglion Neurons in Rats with Chronic Constriction Injury of Sciatic Nerve

目的:观察背根神经节(DRG)磷酸化的p38丝裂原活化蛋白激酶(p -p38MAPK)在大鼠坐骨神经慢性压迫性损伤(CCI)后表达的变化,探讨慢性神经痛的发生机制。方法:2 4只雄性SD大鼠随机分成空白对照组(Naive组)、假手术组(Sham组)、坐骨神经结扎后7d组和1 4d组(CCI7d和CCI1 4d组) ,分别在各自的时间点灌注取材L4- 5背根神经节,用免疫组织化学方法观察磷酸化p38MAPK表达的变化。结果:坐骨神经结扎组背根神经节磷酸化的p38MAPK免疫阳性神经元数和染色深度均明显增加,与假手术组或空白对照组相比,差异具有显著性(P <0 .0 1 )。结论:坐骨神经损伤后背根神经节p38MAPK的激活与神经性疼痛的病理过程密切相关。

Objective:To investigate the changes of phosphorylated p38MAPK expression in DRG neurons in rats with chronic constriction injury (CCI)of sciatic nerve. Methods: Immunocytochemistry were employed to investigate the phosphorylated p38MAPK expression in L_ 4～5 DRG neurons in Rats in CCI d7, d14, nave and sham groups. Results: There was significant increase in numbers and grey level of p-p38MAPK immunoreactive cell in CCI group(compared to nave or sham group, P<0.01). Conclusions: The activation of p38MAPK in DRG in rats contributes to the development of CCI induced-neuropathic pain.