烷烃发酵产生1,13-十三碳二元酸及其诱导作用的研究

PRELIMINARY STUDIES ON THE PRODUCTION OF TRIDECANEDIOIC ACID AND THE INDUCTION IN ALKANE FERMENTATION BY A MUTANT OF CANDIDA TROPICALIS

从能利用正十二烷产生1,12-十二碳二元酸的热带假丝酵母突变株D_(28)出发,经两次紫外线照射诱变,选育到一株从正十三烷产生1,13-十三碳二元酸较高的突变株2-23号菌。该突变株较出发菌株提高产酸率20%,达40.4 g/L。突变株2-23也能将一定链长的长链烷烃以较高的产率转变成相应的单一二元酸。此外,在产酸摇瓶条件试验中观察到烷烃的诱导作用,使突变株产酸能力得以提高。用烷烃预培养的种子发酵正十三烷,其产生1,13-十三碳二元酸的量较糖质碳源培养的种子发酵时要提高30%。

A mutant strain 2—23 derived from the good dodecanedioic acid producer D_(28), a mutant of Candida tropicalis, was obtained through twice treatment with ultra-violet irradiation: The yield Of tridecanedioic acid by the mutant from tridecane reached 40.4 g/l, which was 20% higher than that by its ancestor. Moreover, the strain is able to convert some single long chain alkanes having the number of carbon atoms more than 11 to the corresponding dioic acids with higher yield. The growth of mutant 2—23 on longer chain alkanes led to an induction which promoted the accumulation of tridecanedioic acid from tridecane. A 30% increase in the level of the acid production was observed by the cells grown on alkane as compared to the level by the ones grown on malt extract.