肝癌患者P53、CD34、nm23中的表达与预后的关系

Relations between genic protein expressions of P53, CD34 and nm23 and clinical prognoses of liver cancer

目的 探讨肝癌患者P5 3、CD3 4、nm 2 3基因蛋白表达与临床病理特征及预后的关系。方法 用免疫组化SP法检测5 0例肝癌组织P5 3、nm 2 3、CD3 4的表达,并根据临床病理资料进行各因素的相关性分析。以5 5例癌旁肝组织及非癌性增生肝结节为对照组。分析患者基因蛋白与预后的关系。结果 P5 3、CD3 4在肝癌组织的表达比对照组明显增高,且在有与无门脉瘤栓组间差异有显著性(P <0 .0 5 ) ;nm 2 3基因蛋白则在对照组中表达率增高,而且nm 2 3在癌组织中的表达与显著的瘤周淋巴细胞浸润有关(P <0 .0 5 ) ;不同蛋白组合联合分析生存情况发现,P5 3 (+ )、CD3 4(+ )、nm 2 3(-)组同其他组相比,生存时间明显缩短(P <0 .0 5 )。结论 P5 3、nm 2 3、CD3 4的综合表达对肝癌有重要的辅助诊断价值,并且P5 3突变、CD3 4的高表达可作为肝癌预后不良的可行性指标。

Objective To study the relations between genic protein expressions of P53, CD34 and nm23 and the clinicopathologic characteristics and the prognoses in patients with liver cancer. Methods SP immunostain method was used in the determination of the expressions of P53, CD34 and nm23 in 50 patients with liver cancer and analysis of their correlation based on the clinicopathologic data. 50 patients with HCC/liver and hepatic focal nodular hyperplasia were the control group. Results The expressions of P53 and CD34 in the liver cancer group were much higher than those in the control and there showed significant differences in the grouptumor thrombi in portal vein groups (P<0.05). The genic expression level of nm23 in the control group increased and there existed correlation between the expression of nm23 in cancer cells and the notable tumor lymphocyte infiltration(P<0.05). Coalition analysis of different protein combinations found the survival times of P53(+), CD34(+) and nm23(-)were much shorter than those in other groups (P<0.05). Conclusion The expressions of P53, nm23 and CD34were of important diagnostic value in liver cancer diagnoses and the mutation of P53 and the high expression of CD34 could be used as feasible determinants for bad prognosis of liver cancer.