细菌生长的热谱图测定

DETERMINATION OF THERMOGRAMS OF BACTERIAL GROWTH

细菌细胞内的各种代谢过程都伴有一定的热效应。用微量热法连续测量细菌生长过程中的这种热效应的变化,可获得细菌生长的'热谱图'(thermograph),为细菌代谢和生长特性的研究提供有用的数据。早在60年代,Forrest等就对此法进行过广泛研究。而后Boling等则用此法测得了肠杆菌科的10属17种细菌在脑心浸液培养基中生长的热谱图,对用热谱图鉴别细菌进行了有益的探索。但他们测得的热谱图似不够完整,本文应用微量热法测得了细菌生长的完整的热谱图。

The fundamental growth thermograms of bacteria have been determined by using the mircocalorimetric method. These perfect thermogram curves reflect the changes of bacterial growth patterns (including the lag phase of growth, log growth, stationary phase and the decline phase of growth). In our experiments, highly characteristic and reproducible growth patterns are observed under same condition, therefore one can use these thermograms as 'finger print' to discriminate bacteria. On the other hand, there thermogram curves contain ample imformation, which are very significant for the studies on microorganism metabolism, bio-thermokinetic and clinical fields.