摘要
目的 观察饮用纯净水及加镁纯净水对大鼠肝脏低密度脂蛋白受体 (LDL R)基因表达的影响 ,探讨纯净水致血脂异常的作用机制。方法 健康Wistar大鼠 6 0只 ,随机分为自来水组 (对照组 )、纯净水组和加镁纯净水组(纯净水 +0 5g/LMgSO4,7H2 O) ,每组 2 0只 ,雌雄各半。大鼠饲养至 6个月时处死 ,取肝脏用RT PCR法检测肝脏LDL RmRNA表达量。结果 饮用纯净水可使大鼠肝脏LDL RmRNA表达水平有下降趋势 ,但差异无统计学意义 ;加镁纯净水组大鼠肝脏LDL RmRNA表达水平 ,比单纯饮用纯净水组有上升趋势 ,但差异无统计学意义。结论 观察期内饮用纯净水及加镁纯净水对大鼠肝脏LDL RmRNA表达水平无显著影响。
Objective To investigate the effect of purified water on hepatic expression of low density lipoprofein receptor(LDL R) in rats.Methods 60 male and female Wistar rats were equally randomized into 3 groups〔tap water group(TW),purified water group(PW)and purified water+MgSO 4 group(PW+Mg)〕,Levels of hepatic LDL R mRNA were examined by RT PCR after 6 months treatment.Results Compared with the TW(control group),a decrease trend in LDL R of gene expression in PW and an increase trend in LDL R of gene expression in PW+Mg which compared with the PW were observed,but no differences were seen among all groups.Conclusion Compared with the TW(control group),drinking PW group and PW+Mg group did not obviously affect the levels of hepatic LDL R mRNA in rats.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2005年第3期275-276,共2页
Chinese Journal of Public Health
基金
国家自然科学基金重点资助项目 (5983830 0 )
关键词
纯净水
低密度脂蛋白受体
基因表达
purified water
low density lipoprotein receptor(LDL-R)
gene expression
