乙型肝炎病毒前S1抗原与HBV DNA及HBeAg关系

Detection of hepatitis B virus Pre-S1 antigen in serum and its relationship with HBV DNA and HBeAg

目的 探讨乙型肝炎病毒 (HBV)前S1抗原 (PreS1Ag)与HBVDNA和HBeAg之间的关系 ,以评价其在乙肝诊疗中的作用。方法 用ELISA法对 2 2 5例HBeAg阳性标本进行PreS1Ag和HBV血清标志物的检测 ,用PCR-ELISA法测定HBVDNA。结果 PreS1Ag在HBVDNA和HBsAg阳性组织中的检出率均明显高于相应的阴性组(均为P <0 0 1)。HBeAg阴性组中PreS1Ag的检出率为 4 3 0 % ,PreS1Ag与HBVDNA的符合率为 70 6 % ,与HBeAg的符合率为 6 8 4 %。结论 PreS1Ag与HBVDNA、HBeAg呈良好的相关性。作为反映HBV感染和复制的指标 ,PreS1Ag较HBeAg更敏感。

Objective To investigate the relativity between hepatitis B Virus Pre-S1 Antigen (PreS1Ag) and HBV DNA,HBeAg.And further to judge the value of PreS1Ag detection.Methods In 225 HBsAg positive serum,preS1Ag and HBV marks were detected by enzyme linked immunosorbent assay (ELISA) and HBV DNA was tested by polymerase chain reaction-ELISA (PCR-ELISA).Results The positive rate of PreS1Ag in the two groups of HBV DNA positive and HBeAg positive was obviously higher than that of the negative group;the positive rate of PreS1Ag in the group of HBeAg negative was 43 0%.The coincidence rate of PreS1Ag with HBV DNA and HBeAg were 70 6%,68 4% respectively. Conclusion There are a good relationship among PreS1Ag and HBV DNA,HBeAg.PreS1Ag is more sensitive than HBeAg in judging the infectiion and replication of HBV.