甲醛、三氯乙烯、三氯化铝的细胞联合毒作用

Combination toxicology of formaldehyde,trichloroethylene and alchlor with Chinese Hamster Lung Cells

目的 研究甲醛、三氯乙烯和三氯化铝的联合毒作用类型。方法 噻唑蓝法 (MTT)测定 3种物质各自的半数生长抑制浓度 (IC50 )及混合物 (甲醛、三氯化铝、三氯乙烯 )的IC50 ,根据Finney数学模型和Logistic回归方法判断联合作用类型。结果 甲醛、三氯乙烯、三氯化铝及其混合物质的IC50 分别为 0 . 0 2 2 6 ,0 . 36 8,0 . 5 89和0 . 12 4mg/ml,据Finney法求得Q值为 1 .0 8,Logisitc回归模型参数 β4差异无统计学意义。结论 甲醛、三氯化铝、三氯乙烯 (6∶11∶33)混合染毒 ,对中国仓鼠肺细胞的联合毒性表现为相加作用。

Objective To study the combined action of formaldehyde,trichloroethylene and alchlor.Methods 50% inhibition concentration (IC_ 50) and 95% confidence limits of formaldehyde,trichloroethylene,alchlor and mixture (formaldehyde,trichloriethylene and alchlor) were assessed using Methyl thiazolyl tetrazolium (MTT) with Chinese Hamster Lung Cells.Combined action of above mixture was evaluated according to Finney model and Logistic Regression Method.Results IC_ 50 of formaldehyde,trichloroethylene,alchlor and the mixture were 0.0226mg/ml,0.368mg/ml,0.589mg/ml and 0.124mg/ml respectively.The Q value was 1.08 and Parameter β_4 from Logistic Regression Method showed no significant.Conclusion Combination toxicology of above mixture (formaldehyde:trichloroethylene:alchlor=6∶11∶33) belonged to additive effect.