叶绿酸对恶性转化细胞周期及体外生长的影响

Effects of chlorophyllin on malignant transformation cell cycle and proliferation in vitro in human bronchial epitheial cells

目的 研究叶绿酸对反式 7,8二羟 9,10环氧苯并 (a)芘 (反式BPDE)恶性转化人支气管上皮细胞系(16HBE)体外生长及细胞周期影响。方法 采用噻唑蓝 (MTT)比色法和流式细胞术 (FCM) ,同时观察正常人支气管上皮细胞 (16HBE)、反式 -BPDE恶性转化细胞系、叶绿酸抗反式 -BPDE恶性转化细胞系生长曲线和生长周期的变化以及经叶绿酸处理后的影响。结果 BPDE恶性转化细胞系增殖活性明显大于正常细胞系 (16HBE) ,经 10 0 μmol/L叶绿酸抗BPDE恶性转化细胞组增殖活性较转化组有一定降低。经同样浓度叶绿酸处理的正常细胞组 ,未见降低细胞增殖活性作用。反式 -BPDE恶性转化细胞组G0 /G1期细胞周期比例明显少于正常组细胞。但经叶绿酸抗转化组G0 /G1期比例有所提高 ,而经同样浓度叶绿酸处理的正常细胞组G0 /G1期细胞周期比例无明显改变。结论 对正常细胞无影响的一定浓度叶绿酸有抑制反式 -BPDE恶性转化人支气管上皮细胞增殖活性的作用 ,并通过提高恶性转化细胞G0 /G1期细胞周期比例而影响细胞周期的进程 ,从而改变恶性细胞的增殖和分化。

Objective To study the effects of chlorophyllin on proliferation and cell cycle in vitro in human bronchial epithelial cell line (16HBE) transformed by trans-benzo(a)pyrene-trans-7,8- dihydrodiol-9,10-epoxide (trans-BPDE).Methods MTT method and flow cytometry were used to investigate alteration of the growth curve and cell cycle among untreated control cells,malignant transformed cells induced by trans-BPDE and the anti-transformed cells treated with chlorophyllin and to analyse effects of chloropyllin on the cells.Results Compared with that of control 16HBE,the vitality of malignant transformed cells was higher and it was inhibited significantly in the anti-transformed cells treated with 100μmol/L chloropyllin.However it was uninhibited in the cells treated with only chloropyllin of the same concentration.The proportion of G_0/G_1 cells decreased significantly after being tansformed by trans-BPDE while the proportion increased after being anti-transformed by chlorophyllin.Meanwhile,no change was observed in G_0/G_1 phase of cells treated with the same concentration of chlorophyllin.Conclusion A certain concentration of chlorophyllin may have significant anti-profileration activity in human bronchial epithelial cell line 16HBE.It interfere cell cycle course by enhancing the G_0/G_1 phase proportion in malignant transformed cell and result in change of cell profileration and differentiation.