共轭亚油酸对肥胖大鼠脂肪组织瘦素的影响

Effect of conjugated linoleic acids on leptin level of adipose tissue in obese rats

目的 通过共轭亚油酸 (CLA)对高脂高糖肥胖模型大鼠的干预后 ,观察大鼠体重 ,血脂、以及脂肪组织中的瘦素表达水平的变化 ,探讨CLA对肥胖大鼠影响作用及其机制。方法 选取成年雌性Wistar大鼠 5 0只 ,按体重随机分为 5组 ,1组正常对照组 ,4组模型组 ,模型组高脂高糖饮食 1个月后 ,选出体重大于 2 30 g的大鼠 2 8只 ,在按体重水平随机分为 4组 :肥胖对照组和 3组共轭亚油酸干预组 :低 0 .4 ,中 0 . 8,高 1 6 g/ (kg·bw)。灌胃给予共轭亚油酸4周后 ,测定体重变化和血清中血脂水平 ,Western杂交观察大鼠脂肪组织中瘦素的表达。结果 与肥胖模型对照组相比 ,共轭亚油酸可明显减轻大鼠体重 ,降低大鼠血清甘油三脂水平 ,减少脂肪组织瘦素 (leptin)表达水平。 结论 共轭亚油酸能降低大鼠体重、血脂水平 ,其机制可能是通过调节瘦素表达的来影响脂肪代谢。

Objective To observe the effect of conjugated linoleic acids(CLA)on body weight,Serum lipids level,leptin expression level in obesity rats,and to discuss the mechanism of CLA effect on fat metabolize.Methods 50 female Wistar rats were divided into five groups(normal control group,four obesity model groups).The four group rats were fed with high fat and glucose diets for a month,and body weight>230g 28 rats were divided into four groups(obesity control group,three conjugated linoleic acids groups),and rats were given different conjugated linoleic acids for four weeks later.Body weight,serum lipids level,lpetin expression level were measured.Results Obesity control group,body weight,serum lipids decreased significantly and leptin expression level in adipose tissue were dicreased.Conclusion Conjugated linoleic acids can reduce Body weight,serum lipids level,and the mechanism maybe through intermediation leptin expression level in adipose tissue.