D-半乳糖致氧化损伤小鼠SOD活力及基因表达

Study on SOD gene expression and SOD activity in aging model mice induced by D-galactose

目的 探讨D -半乳糖致氧化损伤模型鼠超氧化物歧化酶 (SOD)活性及其基因表达的相关性。方法 采用生物化学和RT- PCR方法对D -半乳糖造模小鼠、正常对照组、人参蜂王浆口服液组小鼠进行了肝组织SOD活力及SOD基因表达的测定。结果 D -半乳糖造模小鼠SOD活力和基因表达明显降低 ,与对正常照组相比差异有统计学意义 (P <0 . 0 1) ;与D -半乳糖造模组小鼠比较 ,给予不同剂量人参蜂王浆口服液小鼠SOD活力相对升高 ,低、中剂量升高幅度较大 (P <0 . 0 1) ,SOD基因表达也相对上调 ,其中中剂量上调较为明显 (P <0. 0 5 )。结论 D -半乳糖造模小鼠SOD活力降低 ,SOD基因表达也相应降低。人参蜂王浆口服液可减弱D -半乳糖对SOD活力及SOD基因表达的抑制作用。

Objective To investigate the changes of activity and expression of superoxide dismutase(SOD)in aging model mice induced by D-galactose.Methods Kunming mice were randomly divided into three groups including aging model mice induced by D-galactose group,control group and three level of panax(low,middle and high)groups.Biochemical assay and reverse transcription polymerase chain reaction(RT-PCR)were adopted to measure changes of SOD activity and gene expression in different groups.Results Activity and gene expression of SOD decreased significantly in aging model mice induced by D-galactose than those of control mice(P<0.01).Compared with aging model mice group,activity and gene expression of SOD increased in all groups of panax.And the increase was significant in middle level group(P<0.01).Conclusion The activity and gene expression of SOD decreased in aging model mice induced by D-galactose.Oral-intake liquid of ginseng royal jully could lower the decreased of SOD's activity and gene expression.