对肿瘤有抑制作用的假单胞菌新种——济南假单胞菌

A SARCOMA-STATIC NEW SPECIES OF PSEUDOMONAS, PSEUDOMONAS JIN AN ENSIS SP. NOV.

1973年自山东省无影山表土中分离出一株革兰氏阴性杆菌(编号161)。它对小鼠的宫颈癌、肝癌和黑色素瘤有明显抑制作用;对金黄色葡萄球菌、枯草芽孢杆菌和微球菌也有拮抗作用。该菌具极生丛鞭毛。直或稍弯曲的杆状,0.5—0.6×1—2μm。培养2—5d的细胞内含有聚-β-羟基丁酸盐颗粒。产生绿色水溶性色素和绿色荧光色素。营呼吸代谢。化能异养菌。许多含碳的化合物可作为唯一的碳源利用,例如葡萄糖、海藻糖、乙醇、纤维二糖、岩藻糖、精氨酸和甜菜碱。不利用丙酸和酒石酸。不需要生长因子。液化明胶。不水解淀粉。产精氨酸双水解酶。7—37℃和pH 5.65—8.40生长。不能以氢营自养生活。DNA的G+C含量为58.1mol%。它与已知的假单胞菌均不相同。因此,认为菌株161为假单胞菌属的一个新种,命名为济南假单胞菌(Pseudomonas jinanensis Cai & Wang sp.nov.)。

A strain of Gram negative bacteria was isolated from the surface soil of Wuying Hill at Jinan, Shandong province with Gause's medium in 1973. It is a strain of antagonistic bacteria for hysterocervicoma, hepatoma and melanoma of mice screened from 2100 strains of bacteria, It is also antogonistic to Staphylococcus aureus, Bacillus subtilis and Micrococcus.It is a Gram nagative bacterium with lophotrichous polar flagella. Straight rods in shape or with a little slightly curved rods, 0.5—0.6×1—2μm, randomly arranged, polybata-hydroxybutyrate granules are accumlated in cells after 2—5 days cultivation. Water green soluble pigment and green fluorescent pigment are produced. Respiratory metabolism, chemoorganotroph, many carboncontaining organic compounds can be used as carbon sources, such as glucose, trehalose, ethanol, cellulobiose, fucose, arginine and betaine, but propionic acid or tartaric acid is not utilized. Inorganic nitrogen containing compounds can be used ae the sole source of nitrogen. No growth factor is necessary for growth. Gelatin is hydrolyzed. Starch and cellulose are not hydrolyzed. Nitrate is not reduced. Arginine dihydrolase is produced. Levan is produced from sucrose. Growth occurs from 7℃ to 37℃ and from pH 5.65—8.40. No growth occurs at 40℃ and at pH value below 4.86. It can not grow autotrophically with hydrogen. Its G+C contents in DNA is 58.1mol%. DNA-DNA hybridization experiments reveales a relatedness value of 58.6% between this strain and Ps. fluorescens.The above evidence shows that this strain differs from all species known in Pseudomonas, such as Pseudomonas fluorescens group. Pseudomonas caryophylli, Pseudomonas cepacia, Pseudomonas marginata, Pseudomonas acidovorans, Pseudomonas testosteroni and Pseudomonas delafieldii. Therefore we propose it to be a new species in genus Pseudomonas, Pseudomonas jinanensis sp. nov. The type strain is preserved in the General Microbiological Center of China Committee of Culture Collection of Microorganism as AS 1.1765.