根癌土壤杆菌接合转移和生物型特性的遗传学研究

CHROMOSOMAL TRANSFER OF AGROBACTERIUM TUMEFACIENS BY CONJUGATING AND GENETICS STUDY OF BIOTYPE CHARACTERISTICS

用转座子Tn5-Mob和辅助诱动转移质粒R 68.45将根癌土壤杆菌(Agrobactcrium tumefaciens)生物型(Biotype)Ⅱ和Ⅲ接合转移到具三重缺陷的生物型Ⅰ的菌株中,获得了七组转移接合子。通过对其中六组转移接合子生物型分类的主要生理生化特性(3-酮基乳糖反应、石蕊牛奶反应、赤藓糖醇产酸、乙醇产酸、松三糖产酸、粘酸产碱及在New和Keer的选择性生长培养基上能否生长等)测试,发现这些性状通过接合转移而被转移到受体菌中。

By using transposon Tn5-Mob and plasmid R68.45, we transferred the chromosome of Agrobaterium tumefaciens biotype Ⅱ and Ⅲ to biotype Ⅰ strain which has three auxotrophic markers. We got seven groups of trans-conjugats. After analysis of the main classical characteristics of biotypes: 3-ketolactose production; limus milk; acid production from erythirtol, ethanol and melezitose; alkali from mucic acid and growth on selective media New and Keer, we found that these characteristics have transferred to the recipient.