索氏甲烷丝菌的表面结构

THE ULTRASTRUCTURE OF THE SURFACE LAYER OF METHAN OTHRIX SOEHNGENII

用电子显微镜的负染色和冷冻蚀刻方法,观察了索氏甲烷丝菌的表面结构。索氏甲烷丝菌的细胞壁表面可见两种规则排列的结构,靠近细胞壁处是线状排列的蛋白质亚单位,每根线条宽约8—14nm。在线状排列亚单位的上面,是呈四角形排列的蛋白质亚单位,每个亚单位直径约9—14nm。比较了索氏甲烷丝菌与其它产甲烷细菌表面层结构的差异,对表面层蛋白质的特性和功能进行了讨论。同时,对索氏甲烷丝菌细胞质膜的内部结构进行了观察。

The structure of surface layer of Methanothrix soehngenii was examined by electron microscopy of freeze-etching and negative staining preparations. Two types of crystalline protein array can be observed in Methanothrix soehngenii. One consisted of a series of closely arranged striation. Each stiation was about 8 to 12 nm wide. On these striations, there were tetragonally arranged protein subunits. Each subunit diameter was about 9 to 14 nm. The striation structure of cell surface of Methanothrix soehngenii was similar to that of Methanospirillum hungatii and Methanothrix concillii, but tetragonlly arranged subunit of cell surface was entirely different from other all methanogens. This observation showed hat the structure of surface layer of Methanothrix soehngenii was unique and was different from previous reports.