黑曲霉病毒含量与寄主葡萄糖淀粉酶产量之间的相关性

VIRUS OF ASPERGILLUS NIGER AND THE PRODUCTION GLUCOAMYLASE

我国工业上广泛应用的葡萄糖淀粉酶生产菌黑曲霉(Aspergillus nider)体内含有大量病毒。选择在育种筛选中葡萄糖淀粉酶产量不同的菌株,挑选不同方式保藏传代造成葡萄糖淀粉酶产量不同的菌株,以及经化学试剂处理后葡萄糖淀粉酶产量不同的菌株。分別进行病毒提取,电镜观察、免疫双扩散、~3H标记放射免疫测定病毒在体内的滴定度,~3H标记后提取病毒及病毒核酸测定对体內病毒核酸的参入,及PAS-ELISA(酶联A蛋白夹层酶联免疫吸附法Staphyllococcus protein A sandwich ELISA)与SA-test(病毒葡萄球菌共凝集试验Virus staphlococcal co-aggluintion test)检测病毒含量,并且测定了葡萄糖淀粉酶的产量。证实了黑曲霉细胞内病毒的含量与黑曲霉的葡萄糖淀粉酶产量之间呈正相关性。

Iccsahedral virus particles of 26—33 nm in diameter have been found in Aspergillus niger AS 3.4309 an industrial glucoamylase producer. But litter is known about effects of virus on enzyme production. We have examined for the presence of virus in 18 strains and the correlation between the virus concentration and glucoamylase product.Antiserum to virus from A. niger AS 3.4309 was prepared and used in an immune radioactive assay for quantitative determination of virus. Concentration of ~3H labelled virus or viral-dsRNA were determined in mycelia of A. niger parent strain and offspring obtained after being treated with Actinomycin D, ethidium bromide, UV and being storaged in different conditios. Results indicated that the virus titre eas correlated with the glucoamylase product, that is, strains with higher output of enzyme associated with higher concen trations of virus.