一种快速简便检测马铃薯病毒的方法——病毒细菌协同凝集法(VBA)的研究

VIROBACTERIAL AGGLUTINATION TEST(VBA) --A SIMPLE AND RAPID METHOD FOR DETECTING POTATO VIRUSES

在国内，外首次选用金色葡萄球菌的Ｎｏ．１８０菌株用于病毒细菌协同凝集试验检测了来自马铃薯的４种不同形态粒子的病毒：马铃薯Ｘ病毒、马铃薯丫病毒、烟草花叶病毒和马铃薯卷叶病毒，其灵敏度达２．７－６．１ｎｇ／ｍｌ，检出病汁液的最大稀释度达１０＾４～１０＾５（ＲＬＲＶ１：５００），较国内，外采用的Ｃｏｗｎ Ｉ菌株的灵敏度提高５－１０倍，与血清学方法相比，ＶＢＡ在２－３分钟内就可获得结果，无假阳性反应。

Strain No. 1800 of Staphylococcus aureus was first used in Virobacterial agglutination test (VBA) to have detected four types of morphological potato viruses (PVX,PVY,TMV and PLRV). The sensitivities are 5-10 times higher than those from using strain Cown I and Walkey modified method of bacteria cell suspension prepartion, which can be reached 2. 7-6.1ng/ml for purified viruses and 104-105 for infected saps except PLRV (1 : 500). The storage period of cell suspension was not effected on the sensitivities of the VBA and which were to be the highest among IM-ELISA .IEM ,MPT,CIEP and IDD, almost equal to SPA-ELISA. Also , our results have proved that the specificity of the VBA was more high than those of the others. By using VBA and IM - E11SA, 93 infected potato samples form fields were detected and most of the samples were infected by 2-3 viruses (PVX, PVY and PLRV). The results from the indicator plant method were consistent with those from VBA and IM-EL1SA. All of the results have proved that the VBA is a sensitive, specific, rapid, economic, simple and applicable method for detecting viruses of potato or of other plants, especially for the use in basic units.