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Identification of candidate genes for drought stress tolerance in rice by the integration of a genetic (QTL) map with the rice genome physical map 被引量:6

Identification of candidate genes for drought stress tolerance in rice by the integration of a genetic (QTL) map with the rice genome physical map
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摘要 Genetic improvement for drought stress tolerance in rice involves the quantitative nature of the trait, which reflects the additive effects of several genetic loci throughout the genome. Yield components and related traits under stressed and well-water conditions were assayed in mapping populations derived from crosses of Azucena×IR64 and Azucena×Bala. To find the candidate rice genes underlying Quantitative Trait Loci (QTL) in these populations, we conducted in silico analysis of a candidate region flanked by the genetic markers RM212 and RM319 on chromosome 1, proximal to the semi-dwarf (sd1) locus. A total of 175 annotated genes were identified from this region. These included 48 genes annotated by functional homology to known genes, 23 pseudogenes, 24 ab initio predicted genes supported by an alignment match to an EST (Expressed sequence tag) of unknown function, and 80 hypothetical genes predicted solely by ab initio means. Among these, 16 candidate genes could potentially be involved in drought stress response. Genetic improvement for drought stress tolerance in rice involves the quantitative nature of the trait, which reflects the additive effects of several genetic loci throughout the genome. Yield components and related traits under stressed and well-water conditions were assayed in mapping populations derived from crosses of Azucena×IR64 and Azucena×Bala. To find the candidate rice genes underlying Quantitative Trait Loci (QTL) in these populations, we conducted in silico analysis of a candidate region flanked by the genetic markers RM212 and RM319 on chromosome 1, proximal to the semi-dwarf (sd1) locus. A total of 175 annotated genes were identified from this region. These included 48 genes annotated by functional homology to known genes, 23 pseudogenes, 24 ab initio predicted genes supported by an alignment match to an EST (Expressed sequence tag) of unknown function, and 80 hypothetical genes predicted solely by ab initio means. Among these, 16 candidate genes could potentially be involved in drought stress response.
出处 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE EI CAS CSCD 2005年第5期382-388,共7页 浙江大学学报(英文版)B辑(生物医学与生物技术)
基金 Project supported partly by the Rockefeller Foundation thesis dis-sertation training grant and the National Hi-Tech Research and De-velopment Program (863) of China
关键词 基因 染色体 耐旱性 稳定性 Rice genome sequence, Candidate genes, Drought stress, Quantitative Trait Loci (QTL)
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  • 1Z. K. Li,S. B. Yu,H. R. Lafitte,N. Huang,B. Courtois,S. Hittalmani,C. H. M. Vijayakumar,G. F. Liu,G. C. Wang,H. E. Shashidhar,J. Y. Zhuang,K. L. Zheng,V. P. Singh,J. S. Sidhu,S. Srivantaneeyakul,G. S. Khush.QTL × environment interactions in rice. I. Heading date and plant height[J].Theoretical and Applied Genetics.2003(1)
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