巨细胞病毒冻干保存

PRESERVATION OF CYTOMEGALOVIRUS BY LYOPHILIZATION

巨细胞病毒(Cyromegaloviyus,CMY)是疱疹病毒科的成员,人类及其它一些哺乳动物易受该病毒感染,于怀孕、多次输血及器官移植时可激活,造成显性感染。孕妇感染对胎儿危害较大,是人类先天性畸形的重要病原之一。近年来,随着国内外医学界对该病毒的广泛研究,要求提供毒种进行研究的单位日益增多,但由于该病毒在培养过程中繁殖缓慢而且不易保存,一般都沿用液氮为主要保存手段。

The preservation of cytomegalovirus (CMV) by lyophilization was first developed in China. In 4 to 10 days the typical CPE was up to ++++ in the HEL cell line which was inoculated only 0.2ml suspension of lyophilized strain AD_(169) or CMV isolated from milk breast. After preserved for 1 year the lyophilized cytomegalovirus strain still contained 1.5—2.0lgTCID_(50)/0.1ml in titer of viral infection. The lyophilized strains have been used in the preparation of diagnostic antigen of cytomegalovirus in our department, also the strains were supplied to the whole country.