摘要
通过对棕色固氮菌(Azotobacter vinelandii)沈-230菌株在不同时间培养下,菌的生长、繁殖与固氮酶(N_2ase)、过氧化物酶(PER)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)的活力变化研究,发现棕色固氮菌中PER、CAT、SOD的活力水平与N_2ase活性变化有密切的相关性。在对数生长期,N_2ase活性随生长时间的延长而增加,同时CAT、PER和SOD酶的活性也相应增加。对数期之后,N_2ase活力稍有下降,其它酶的活力亦有相应的变化。在研究酶活力变化的同时,研究了同工酶谱的变化。该变化亦有很强的规律性。根据研究结果,提出并讨论了棕色固氮菌中固氮酶的防氧保护机制。
Oxygen is potentially toxic to living cells. Aerobic organisms have evolved an enzymatic mechanism against oxygen damage. Azotobacter vinelandii 230 is aerobic bacterium. Under the different period of culture time, the activities of catalase, peroxidase and superoxide dismutase have close relation with that of nitrogenase. Before log-growth phase, the activity of nitrogenase increase with culture time, and at the same time the activities of the other three enzymes also increase. After the log-growth phase, the nitrogen-fixing activity decrease slightly, and the other three enzymes have similar characteristics.
In this paper, the characteristics of peroxidase is also described.
出处
《微生物学报》
CAS
CSCD
北大核心
1989年第6期439-443,共5页
Acta Microbiologica Sinica
基金
国家科学基金
关键词
棕色固氮菌
固氮酶
过氧化物酶
Nitrogenase
Peroxidase
Catalase
Superoxise dismutase
Azotobacter vinelandii
