戊二醛鞣制的牛颈静脉带瓣管道钙化的实验研究

Research on calcification feature of bovine jugular vein conduit cross-linked by glutaraldehyde

目的探讨戊二醛鞣制的牛颈静脉带瓣管道是否易于钙化。方法本研究以戊二醛鞣制的牛颈静脉作为研究对象,通过建立大鼠皮下包埋动物模型11例加速体内钙化的方法,应用原子吸收光谱法测定组织钙含量。结果血管壁和瓣膜钙含量分别为(211.00±25.83)μg/m g和(4.218±3.879)μg/m g,后者为前者的2%,统计学上差异有显著性(P<0.001)。结论戊二醛交联鞣制的牛颈静脉血管壁较易发生钙化,与之相比瓣膜较不易钙化。

To research the calcification feature of bovine jugular vein conduit cross-linked by glutaraldehyde. Fresh bovine jugular vein was cross-linked by glutaraldehyde. They was cut into pieces and implanted subcutaneously in weanling SD rats, total 11 animals. After 90 days, all the rats were sacrificed and calcium content was determined by flame atomic absorption spectrophotometer. After 90 days implantation, the results showed that the walls and valves calcium content were (211.00±25.83) μg/mg and (4.218±3.879) μg/mg measured by dry weight respectively. The wall of the bovine jugular vein had more calcium than the valves. [Conclusions] The wall of the bovine jugular vein cross-linked by glutaraldehyde is easy to lead calcification and the valve is not.