侵染苜蓿的豇豆花叶病毒的研究

STUDIES OF COWPEA MOSAIC VIRUS ON ALFALFA

从新疆苜蓿矮缩花叶病株上分离到一种病毒分离物G—14,在测定的9科40种植物中,此分离物可侵染6科19种植物,易由汁液摩擦接种,桃蚜(Myzus persicae)、豆蚜(Aphis craccivora)、棉黑蚜(A.atrata)和苜蓿无网长管蚜(Acyrthosiphon rondoi)等不传毒,可由首蓿叶象岬(Hypera variabilis)传毒;致死温度55～60℃,稀释限点10^(-3)～10^(-4),体外保毒期21天;病毒粒体球状,直径约28nm。电镜观察病组织超薄切片,可见晶格状排列的病毒粒体。经琼脂双扩散血清学测定,该分离物与豇豆花叶病毒(CPMV)抗血清呈阳性反应。由此鉴定G—14为CPMV。提纯病毒经SDS—聚丙烯酰胺凝胶电泳测定有3个蛋白组分。分子量分别为44800,24500,22400道尔顿;琼脂糖电泳测得有二个核酸组分。

The isolate G-14 was obtained from infected alfalfa plants showing dwarf mosaic symptoms in Xingjiang. It infected 19 of 40 species from 6 of 9 families by sap inoculation. The isolate was transmitted by beetle Hypera variabilis but not by aphids. TIP = 55~ 60℃, DEP = 10-3- 10-4, LIV = 21 days. The viral particle was isometric,about 28 nm in diameter. The lattace - like inclusions which arrange in good order were found in leaf tissue of infected alfalfa plants. This isolate reacted with the antiserum of CPMV in agar gel double diffusion test, and has three protein components of different ML. Wt. , each with two nucleic acid components. This isolate G-14 of alfalfa is demonstrated to be CPMV.