小麦条锈菌夏孢子阶段核相状况的研究

NUCLEAL CONDITION OF UREDINIAL STAGE OF WHEAT STRIPE RUST

本文应用电镜和荧光染色技术对小麦条锈菌夏孢子阶段的细胞核相状况进行了系统研究。观察发现夏孢子通常为双核体,夏孢子萌发的芽管可为双核、三核或四核,但仍以双核芽管为主。尽管在胞间菌丝和吸器母细胞中可观察到典型的双核细胞,但胞间菌丝和吸器母细胞中的多核现象极为普遍。经连续切片观察证实,吸器的细胞核可为双核、三核、四核、五核和六核,一般以四核为主。夏孢子堆中的不同类型的细胞均为双核。上述研究结果表明,小麦条锈菌的细胞核相状况较为复杂,并且显然不向于其它锈菌。关于小麦条锈菌多核现象的生物学意义值得进一步研究。

The nucleal condition of uredinial stage of wheat stripe rust was studied with electron and flouresence microscopy. It was found that urediniospore usually contained two nuclei, while the germ tube from urediospore possessed two,three or four nuclei and germ tube with two nuclei was dominant. The multinuclea) condition in intercellular hyphae and Haustoria mother Cell was very common although intercellular hyphae and Haustoria mother cell with two nuclei could be observed. The observation from serial sections showed that there were two,three,four,five or six nuclei in Haustoria,in which four nuclei was dominant. All types of cell in uredinia were found to be binuclei. The results here obviously indicated that the nucleal condition of wheat stripe rust was complicated and different from that of other rust.