毛细管电泳测定肝癌肝硬化患者血清氨基酸的临床意义

Analysis of Serum Amino-acid in the Diagnosis of Liver Cancer and Liver Cirrhosis with Capillary Electrophoresis

目的 用毛细管电泳的方法测定肝癌肝硬化患者血清氨基酸变化,并探讨其临床意义。方法 用P/ACEMDQ毛细管电泳仪对2 0例正常人、2 0例肝癌患者及2 0例肝硬化患者的血浆游离氨基酸进行测定。结果 在9min内分离12种氨基酸,线性在2 0～10 0 0 μmol/L范围,回收率为77. 5 %～113 .3% ,批内精密度2 . 6 %～11. 4 % ,批间精密度5 . 8%～19. 7%。对结果进行统计学分析,发现肝硬化组酪氨酸、丝氨酸、色氨酸明显升高,丙氨酸明显下降;肝癌组酪氨酸、半胱氨酸、缬氨酸明显升高,脯氨酸明显下降。结论 毛细管电泳测定血浆游离氨基酸对肝癌肝硬化的诊断和治疗有指导意义。

Objective To study the concentrations of the ch a nges of serum free amino acids in liver cirrhosis patients and liver cancer pati ents.Methods The assay was based on percolum derivatizati on of s with 2'4-dinitrofluorbenzen(DNFB),separation of the derivatives by h igh performance capillary electrophoresis(HPCE),and quantification by ultraviole t detection.Results The complete separation of 12 kinds o f s was achieved within 9 min.The assay was linear from 20 to 1 000 μmol/L.Th e recovery of s added to serum samples was 77.5%～113.3%,within-run precisi on was 2.6%～11.4%,and between-run precision was 5.8%～19.7%.Compared with the normal,the concentrations of s inside serum in cases of liver cirrhosis a nd liver cancer changed significantly(P<0.05).Conclusion It indicates that the analysis of the serum free s has important value f or clinical diagnosis and cure treatment.