重组人白细胞介素-15包涵体离子交换柱复性与稀释复性的比较

Comparison of protein refolding by chromatographic method and by dilution method

目的比较重组人白细胞介素-15 (rhIL-15 )包涵体蛋白稀释复性法与离子交换柱复性法的效果。方法采用离子交换柱吸附变性包涵体蛋白,用含有复性剂的洗脱液洗脱复性,所得蛋白质比活和稀释复性法所得比活比较。结果两种方法所得目的蛋白质比活相近,但离子交换柱复性法目的蛋白质回收率高,试剂消耗少。结论对于rhIL-15包涵体,离子交换柱复性法用于生产。

PurposeTo compare the protein refolding effect by chromatographic method and by dilution method.MethodsInclusion bodies protein was absorbed with ion exchange column,and then eluting with elution solution containing refolding agents. The specific activity of refolding proteins obtained from the two methods was judged.ResultsAlthough the specific activity of refolding proteins was alike, the target protein recovery rate by chromatographic method was higher than that of dilution method and consumption was lower.ConclusionThe recovery rate of chromatographic method for rhIL-15 inclusion bodies aim protein was higher than that of dilution method,and reagents consumption decreased. This technique could markedly reduce the cost of production.