碱性成纤维细胞生长因子与肿瘤相关物质群的检测在急性白血病中的临床应用

Clinical Application of Basic Fibroblast Growth Factor and Tumor Supplied Group of Factors in Patients with Acute Leukemia

目的探讨急性白血病(AL)患者血清碱性成纤维细胞生长因子(bFGF)及恶性肿瘤相关物质群(TSGF)的水平及其临床意义。方法应用酶联免疫法(ELISA)及化学显色法分别对47例AL初治患者、8例复发患者化疗前及化疗后11例完全缓解(CR)患者、13例难治(NR)患者血清bFGF及TSGF的水平分别进行检测,并与正常对照组比较。结果AL初治组及复发组血清bFGF水平均明显高于正常人对照组水平,差异有非常显著性意义(t=6.71,6.18,P<0.001);AL初治组及复发组血清TSGF水平均明显高于正常人对照组水平,差异非常显著(t=6.847,5.009,P<0.001);CR患者血清bFGF水平及TSGF水平均明显下降,与正常对照组相比较,差异均无显著性意义(t=1.11,1.482,P>0.05);急性淋巴细胞白血病(ALL)NR组化疗前血清bFGF水平明显高于CR组化疗前的水平,差异具有显著性意义(t=2.43,P<0.05),而急性髓细胞性白血病(AML)NR组与CR组化疗前血清bFGF水平无显著性差异(t=0.76,P>0.05);ALL与AML患者NR组化疗前血清TSGF水平均高于CR组化疗前的水平,且差异均具有统计学意义(t=3.29,2.13,P<0.01,0.05);血清bFGF水平与TSGF水平呈正相关(r=0.5263,P<0.01)。结论联合检测血清bFGF及TSGF水平对了解AL的发生、发展及疗效观察有一定临床价值。

Objective To study Serum levels of basic fibroblast growth factor (bFGF) and tumor supplied group of factors (TSGF) in patients with acute leukemia (AL) and their clinical significance. Methods In the study, 47 patients and 8 relapsing patients with AL before treatment, and 11 complete remission (CR) patients and 13 non-remission (NR) patients after treatment were selected. Serum levels of bFGF and TSGF were measured and compared with those of normal controls. Results Serum levels of bFGF in patients with AL before treatment and those in relapsing patients were significantly higher than that in normal controls (t=6.71, 6.18, P<0.001); serum levels of TSGF in patients with AL before treatment and those in relapsing patients were significantly higher than those in normal controls (t=6.847, 5.009, P<0.001). Serum bFGF levels and TSGF levels of CR patients decreased significantly, and the difference between CR group and normal group was not significant (t=1.11, 1.482, P>0.05). Serum bFGF levels of NR acute lymphocyte leukemia (ALL) patients were significantly higher than that of CR group, and the difference between them was significant (t=2.43, P<0.05); in acute myelogenous leukemia (AML) patients, the difference in serum bFGF level between NR group and CR group was not significant (t=0.76, P>0.05). Serum TSGF levels of NR group were higher obviously than those of CR group in both ALL and AML patients (t=3.29, P<0.01; t=2.13, P<0.05). Positive correlation were shown between serum bFGF and TSGF (r=0.5263, P<0.01). Conclusion Serum bFGF and TSGF can be used for detecting clinical features and evaluating curative effect in patients with AL.