以间接碘标记法制备瘦素标记抗原的研究

Sutdy on Preparation of Labelled Leptin Antigen with Indirect Iodination

目的:以间接碘标记法制备瘦素(leptin)的标记抗原(125I-leptin),建立瘦素的放射免疫分析(RIA)并用于临床。方法:先以氯胺T(ch-T)法标记对羟基苯丙酸琥珀酰亚胺脂(Bolton-hunter试剂,BH试剂)制备125I-BH,然后于冰浴中与leptin进行联结制备125I-leptin。结果:125I-leptin的放射性比活度为1 43MBq/μg,125I的总标记率为37 8%,用以建立的RIA各项技术指标均满足临床要求。结论:由于以间接法制备125I-leptin,减少了leptin与氧化剂、还原剂和放射性碘的接触,避免了对其抗原性的损伤,从而制备出具较高标记率和较高放射性比活度的125I-leptin。

Objective To develop method of RIA of leptin for clinical use with 125 I-leptin prepared with indirect iodination. Methods BH reagent was iodinated by ch-T, then conjugated with leptin in ice bath. Results The specific radioactivity of 125 I-leptin obtained was 1.43 MBq/μg, the total labelling rate of 125 I was 37.8%, and the technical specifications of leptin RIA could meet the requirement for clinical use. Conclusion 125 I-leptin with higher total labelling rate and specific radioactivity was prepared, lessening the possible harm of direct iodination and other chemicals to the leptin antigen.