2型糖尿病患者血清脂联素、胰岛素、C-反应蛋白、瘦素水平变化分析

Study on the Changes of Serum Adiponectin (APN), Insulin, C-reactive Protein (CRP) and Leptin Levels After one Year Treatment in Patients with Type 2 Diabetes

目的:通过对2型糖尿病患者血清中脂联素(APN)、胰岛素、C-反应蛋白、瘦素(Leptin)、胰岛素抗体及谷氨酸脱羧酶抗体(GAD-Ab)检测分析,探讨胰岛β细胞功能和胰岛素抵抗的机理,为其早期诊断治疗提供依据。方法:用化学发光、酶联免疫分析、放射免疫分析对184例2型糖尿病患者和30例正常对照者及75例DM2治疗观察组经一年治疗前后水平检测比较分析。结果:正常对照组与DM2比较胰岛素、瘦素、C-反应蛋白、胰岛素抗体有显著性差异(P<0 01),且呈正相关,与脂联素比较有显著性差异(P<0 001)但呈负相关;75例治疗后除脂联素、胰岛素抗体外其它几项指标较治疗前明显下降,有统计学意义,而脂联素较治疗前明显升高(P<0 001)有显著性差异。结论: 2型糖尿病标志物的检测,对其早期诊断、治疗预后及生理机制的研究具有重要意义。

Objective To investigate the β-cell function status and possible mechanism of insulin resistance in patients with type 2 diabetes through studies on the changes of serum APN, insulin, CRP, leptin, insulin antibody and glutamic acid decarboxy lase antibody (GAD-Ab) levels after one year of treatment. Methods Serum levels of the above four parameters and the positive rate of the two antibodies were measured (with CLIA, ELISA and RIA as appropriately) in 184 patients with type 2 diabetes and 30 controls as well as in 75 patients after one year of treatment. Results The serum contents of insulin, leptin, CRP, insulin antibody in patients with type 2 diabetes were significantly higher (P<0.01) and APN levels significanlty lower (P<0.001) than those in controls. Levels of APN were negatively correlated with those of the other parameters. In the 75 treated patients, levels of those parameters (with the exception of APN and insulin-antibody) decreased significantly、 However, the APN levels were significantly increased (vs before treatment, P<0.001). Conclusion Further study on the dynamic changes of these parameters in the diabetic patients might elucidate certain key-points in the pathogenesis of the disease.