支气管哮喘患儿内皮素-1及相关炎性细胞因子测定

Determination of Blood ET-1 and Related Cytokines in Pediatric Patients with Bronchial Asthma

目的:探讨内皮素及相关炎性细胞因子在小儿哮喘发病机制中的作用。方法:分别用放射免疫分析及酶免法测定了42例哮喘患儿治疗前后内皮素-1、白细胞介素-5、白细胞介素-6及白细胞介素-8水平。结果:发作期组内皮素水平高于缓解组及对照组差异非常显著(P值均<0 01),缓解组较发作组水平下降显著但仍显著高于对照组(P<0 05)。3种细胞因子水平则发作期组均显著高于缓解组及对照组,统计差异极显著(P值均<0 01),缓解组前2种细胞因子仍显著高于对照组(P值均<0 05);而后一种细胞因子已下降至近对照组水平(P>0 05)。ET与IL-5呈显著正相关(发作期r=0 560,P<0 01;缓解期r=0 435,P<0 01)。结论:内皮素与细胞因子以不同方式参与了小儿哮喘的发病机制,其测定对于评价炎症程度及疗效、指导临床治疗有重要意义。

Objective To explore the role of endothelin and related cytokines (IL-5, IL-6, IL-8) in the development of bronchial asthma in pediatric patients. Methods Plasma ET-1 (with RIA) and serum IL-5 (with ELISA), IL-6, IL-8 (with RIA) levels were measured in 42 pediatric patients with bronchial asthma and 30 controls. Results The plasma ET-1 levels in the patients during acute attack (n=22) were significantly higher than those in patients during remission (n=20) as well as in controls (P<0.01). Levels in patients during remission were still significantly higher than those in controls (P<0.05). Serum levels of IL-5, IL-6, IL-8 during attack were all significantly higher than those in patients during remission and controls (P<0.01). During remission, levels of IL-5 and IL-6 were still significantly higher than those in controls (P<0.05); however, levels of IL-8 were not much higher than those in controls (P>0.05). Levels of ET-1 were positively correlated with those of IL-5 (during attack, r=0.560, P<0.01; during remission, r=0.435, P<0.01). Conclusion ET and the cytokines IL-5, IL-6, IL-8 participated in the pathogenesis of bronchial asthma through different mechanisms and dynamic determination of which could reflect the progression of the disease.