摘要
目的通过建立血清hK2 ELISA检测方法,探讨血清hK2水平对前列腺癌的诊断意义,为临床鉴别前列腺癌与前列腺增生提供一种特异、敏感、实用的方法.方法利用hK2单克隆抗体建立检测血清hK2水平的ELISA方法,并利用本实验建立的方法对30例前列腺癌患者,26例前列腺增生患者,30例正常对照的血清进行hK2水平的检测.结果 1)血清hK2水平测定值(A450nm)分别为:a.前列腺癌组(0.701±0.113);b.前列腺增生组(0.212±0.145);c.正常对照组(0.198±0.015).前列腺癌组血清hK2水平与前列腺增生、正常对照组相比较有显著差异(P<0.01).2)本研究建立的检测血清hK2水平的ELISA方法的敏感度为83.3%,特异度为94.6%,准确度为87.2%.结论 1)前列腺癌患者血清hK2水平与前列腺增生患者及正常人血清hK2水平差异显著,不存在重叠.血清hK2水平能够对前列腺癌、前列腺增生进行鉴别诊断.2)在鉴别前列腺癌和前列腺增生方面,本研究建立的检测血清hK2水平的ELISA方法,与临床上应用的病理学诊断方法符合率高,具有临床实用价值.
Objective To investigate the serum level's sense for distinguishing prostate carcinoma from prostate proliferation by establishing ELISA detection method for serum hK2, to provide a specific, sensitive, practical method for distinguishing prostate carcinoma from prostate proliferation.Methods We established the ELISA which detect serum hK2 level by using hK2 monoclonal antibody. Using established methods, we detected the serum hK2 level of 30 patients with prostate carcinoma, 26 patients with prostate proliferation and 30 cases of normal controls.Results The level of hK2 are as follows: 1)The prostate carcinoma group((0.701)±(0.113)); 2)The prostate proliferation group((0.212)±(0.145)); 3)The normal control group((0.198)±(0.015)). There are differences among the prostate carcinoma group, prostate proliferation group and normal control group in hK2 level(P<(0.01)).Conclusion There are significant differences comparing the prostate carcinoma patients with prostate proliferation patients and normal control in hK2 level.
出处
《北华大学学报(自然科学版)》
CAS
2005年第2期125-128,共4页
Journal of Beihua University(Natural Science)
基金
吉林市科学技术局科技项目(批准号:2003 02022)