模拟60m空气潜水引起大鼠的氧化应激状态

Oxidative stress in rats induced by simulated air repetitive divings

目的观察模拟60m空气潜水引起大鼠的氧化应激状态。方法将大鼠置于动物加压舱内,暴露于700kPa压缩空气下60min,每天2次,连续3d。于暴露后第1、3、5天915断头取血。用化学比色法测定循环血浆中超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽转硫酶的活力和谷胱甘肽、丙二醛的含量。结果经高压空气多次暴露后,大鼠血浆抗氧化酶活力显著降低(P<0.05或P<0.01),但在暴露后3～5d全部恢复正常;谷胱甘肽含量在暴露后第3天时明显降低(P<0.01),第5天恢复正常;丙二醛含量在多次暴露后显著增高(P<0.01),第5天时降至正常水平。147kPa纯氧暴露后各指标变化类似于700kPa空气暴露。常氧高氮处理后各指标无显著改变。结论60m模拟空气潜水引起大鼠明显的氧化应激,但能在3～5d内恢复;上述变化可能与压缩空气中的高分压氧有关。

Objective To explore the status of oxidative stress in rats caused by 60 m simulated air repetitive divings.Methods Adult rats were placed in an animal chamber (700 kPa air, 60 min) twice daily for 3 days. Peripheral blood was sampled 1, 3 and 5 days after the final exposure. Activities of superoxide dismutase, catalase, glutathione peroxidase, gluta-~thione S -transferase and levels of glutathione, malondialdehyde in plasma were determined colorimetrily. Results After hyperbaric exposures, the activities of anti-oxidative enzymes were decreased significantly (P<0.05 or P<0.01), but these could be recovered in 3-5 days. Glutathione concentration decreased (P<0.01) on the 3rd day after exposures, and reached to normal level on the 5th day. The level of malondialdehyde increased (P<0.01) after exposures and was recovered on the 5th day. The effects of 147 kPa oxygen were similar to those of 700 kPa air. There were no significant changes after treatment with normoxic hyperbaric nitrogen. Conclusion Rats suffered severe oxidative stress from 60 m simulated air repetitive divings, which was caused mainly by the high partial pressure of oxygen in compressed air and could be recovered in 3-5 days.