S-生物烯丙菊酯亚慢性经口毒性和致突变性研究

Experimental research on subchronic oral toxicities and mutagenicities of S-bioallethrin

目的研究农药S生物烯丙菊酯的致突变性和亚慢性经口毒性。方法按GB156701995的方法进行Ames试验、小鼠骨髓微核试验、小鼠睾丸精母细胞染色体畸变试验和大鼠亚慢性经口毒性试验。结果Ames试验各条件下均为阴性(0.5、5、50、500、5000μg/皿)。S生物烯丙菊酯2～40mg/kg剂量组骨髓微核细胞率和10～40mg/kg剂量组睾丸精母细胞染色体畸变率与阴性对照组比较差异均无显著性。亚慢性经口毒性试验中,各剂量组(2.9～38.5mg·kg-1·d-1)在一般状态、体重、脏器系数等指标均基本正常;高剂量组动物和中剂量组动物部分脏器(脑、心、肝、肾、肺、脾、睾丸)有不同程度的病理表现;经综合分析,S生物烯丙菊酯在本试验对SD大鼠亚慢性经口的最大无作用剂量为2.9mg·kg-1·d-1。结论本受试物在本试验剂量下无致突变作用,亚慢性经口的最大无作用剂量可为本农药安全性评价和进一步试验提供依据。

Objective To study the mutagenicities and subchronic oral toxicities of the pesticide S-bioallethrin. Methods Based on the National Standard GB15670-1995, Ames test , bone marrow micronucleus test in mice , chromosome aberration test in mice testicle spermatocytes and subchronic oral toxicity test in rats were conducted. Results A negative result was observed in Ames test at all dose groups (0.5, 5, 50, 500, 5000 μg per utensil), compared with the controlled. No significant differences were observed in micronuclei and chromosome aberration between the controlled and 2~40 mg/kg groups in bone marrow micronucleus test and chromosome aberration test. In the course of the subchronic test , no obvious toxic response was observed in the treated animals. Histopathological examinations revealed that various changes were found in the high and medium dose group (brain, heart, liver, kidney, lung, spleen, spermary). There were no obvious pathological changes in the low-dose treated animals. The 180-day oral no observed adverse effect level (NOAEL) of S-bioallethrin in SD rats was 2.9 mg·kg^-1 ·d^-1 . Conclusion S-bioallethrin is regarded as a non mutagenic pesticide under the tested doses. The NOAEL of S-bioallethrin can be used to evaluate the safety of the pesticide and to provide basis for further studies.