摘要
豆科植物百脉根（ＬｏｔｕｓｃｏｒｎｉｃｏｆａｔｕｓＬ．）是一种优良的牧草。１０ｋＤ玉米醇溶蛋白是一种富硫蛋白，依分子数计算，含硫氨基酸占总氨基酸量的２５％。通过根癌农杆菌（Ａｇｒｏｂａｃｔｅｒｉｕｍｔｕｍｅｆａｃｉｅｎｓ）的介导，将ｒｂｃＳ启动子及ＣａＭＶ３５Ｓ启动于调控下的１０ｋＤ玉米醇溶蛋白基因的嵌合质粒导入百脉根，得到转化的植株，其卡那霉素的抗性由ＢＮＰＴⅡ活性分析进一步得到证明。Ｓｏｕｔｈｅｒｎｂｌｏｔ分析表明，１０ｋＤ玉米醇溶蛋白基因已整合到百脉根的核基因组中。
Lotus corniculatus L. is a common legume forage. 10 kD zein is a maize storage proteinin which about 25% of total amino acids contain sulphur. The chimeric plasmids of the 10kD zein gene regulated by rbcS ;)romoter and CaMV 35S promoter were introduced into L.corniculatus by Agrobacterium tume faciens mediated procedures, and the transformed plantshave been obtained. Kanamycin-resistance of plantlets was evidenced by the presence of enzyme activity from NPT assay. Southern blotting indicated that the 10 kD zein gene hadbeen integrated into the chromosomal genome of L. corniculatus.
基金
中国科学院重大项目基金
关键词
玉米醇溶蛋白
百永根
转基因植株
10 kD zein. Agrobacterium tumefachas: Lotus crmiculatus
Transgenicplant
