醋酸锌对大鼠胚胎肢芽细胞增殖和分化影响的研究

Effect of Zinc Acetate on Proliferation and differentiation of Rat Embryonic Limb Bud Cell

目的 研究醋酸锌对原代培养的大鼠胚胎肢芽细胞形态、细胞相对蛋白含量、细胞集落形成率以及肢芽细胞生长发育的影响。方法 取SD大鼠胚胎肢芽组织,分别加入不同浓度的醋酸锌进行体外原代微团培养。用光学显微镜观察细胞形态,在倒置相差显微镜下计数细胞集落数,同时用Folin-酚法检测细胞蛋白含量。结果 当锌浓度为0.05mmol/L时,对肢芽细胞生长和集落的形成无显著影响,而当锌浓度增至0.1mmol/L时,表现出显著促进作用,锌浓度大于1.0mmol/L时则呈现抑制作用,且影响的程度随着锌浓度的增加而增强。醋酸锌对大鼠胚胎肢芽细胞的半数分化抑制浓度(ICD50)为1360.9μg/mL。结论 醋酸锌对胚胎肢芽细胞增殖和分化的影响呈现双向效应,即低浓度促进,高浓度抑制。

Objective: To study the effect of zinc on embryo, the growth of limb bud cell was observed in vitro by counting the number of colony in culture and detecting the level of cellular protein. Methods: Embryonic cells from 14-day SD rats were primarily cultured in 96 well plate with different concentrations of zinc acetate. Amount of cellular colony was counted under phase-contrast microscope, the level of cellular protein was detected using Folin-phenol method and the cell-morphology was observed with microscope. Results: 0.1mmol/L of zinc promoted the proliferation of limb bud cells and the colony forming, But more than 1.0mmol/L of zinc showed the inhibiting effect. The concentration of zinc to inhibit the formation of differentiation foci by 50% of the control (ICD 50) was shown to be 1360.9μg/mL. Conclusion: The condign dose of zinc can promote the growth of limb bud cell, but over-dose of zinc displayed an inhibited effect.