兰州百合生殖器官中睾酮含量的变化

THE CHANGES OF STEROIDAL SEX HORMONE──TESTOSTERONE CONTENTS IN REPRODUCTIVEORGANS OF LILIUM DAVIDII DUCH.

用直接竞争酶联免疫吸附分析法检测了兰州百合（ＬｉｌｉｕｍｄａｎｄｉｉＤｕｃｈ．）花粉、雄蕊、雌蕊及花瓣中甾类激素──睾酮的含量。雄蕊中睾酮的含量随发育进程上升，开花当天达到最高值。雌蕊和花瓣中的睾酮含量低，而且不伴随花的发育进程变化。生殖器官中睾酮含量以成熟花粉为最高。无论蕾期授粉（亲和）或自花授粉（不亲和）与不授粉的对照相比，雌蕊的睾酮含量无显著差别。百合花粉水合后睾酮含量略有下降。花粉在体外萌发过程中，花粉管中的睾酮含量随花粉管的生长而急剧下降，这时萌发介质中只出现极微量的睾酮，而且在整个萌发时期无明显变化。推测睾酮参与雄配子体的发育和成熟，并与花粉的萌发有一定关系，但无迹象表明与受精的选择性有关。

Testosterone, a steroidal sex hormone, was determined in petal, anther, pistil andpollen of Lilium davidii', using a highly sensitive and specific enzyme linked immunosolventassay (ELISA), and found to be present at levels of 'g/g dry wt. In anther of lily, the levelof testosterone increased during anther development, reached maximum at anthesis. Afterpollen shedding, testosterone rapidly decreased. No obvious changes of testosterone wereobserved with development of pistil and petal. Lily expresses gametophytic self-incompati-bility. The levels of testosterone showed little difference in pistil, either after bud pollination, or self pollination. After hydration, the contents of testosterone in pollen slightly decreased. It rapidly decreased in pollen tube during germination at the first 3 h, but very littleamount was found in germination medium. These results suggest that testosterone is associated with development and ripening of male gametophyte and pollen germination. but nosign indicates its participation in the process of self-incompatibility.