摘要
风信子(HyacinthusorientalisL.)性器官分化和性细胞分化所需条件是不一样的,适合于性细胞(雌配子体)分化的外源激素水平大大低于性器官(胚珠)分化的外源激素水平。珠心细胞在低浓度的外源激素条件下可以分化性细胞;稍稍提高外源激素浓度,性细胞化化停止,代之以珠心细胞的增殖;进一步提高外源激素浓度,珠心细胞又可以分化出新的胚珠原基。
Studies on the effects of exogenous hormones on ovule regeneration and its sexual cell differentiation in Hyacinthus orientalis L.have reveased that conditions required for differentiations of sexual organs and sexual cells were different.The exogenous hormone level suitable for the sexual cell differentiation was greatly lower than that for sexual organ differentiation.The nucellus cells could differentiate the sexual cell under quite low concentration of exogenous hormones.With slight increase of hormone concentration, the differentiation of the sexual cells stopped and proliferation of the nucellus cells took place instead.Upon further increase of the exogenous hormone concentration, new ovule primordia could be formed from the proliferated nucellus cells, which further developed into many new ovules.
基金
国家自然科学基金
关键词
风信子
离体培养
胚珠再生
性细胞
Hyacinthus orientalis L.
In vitro
Ovule regeneration
Sexual cell differentiation