甘蔗原生质体的植株再生

PLANT REGENERATION FROM PROTOPLASTS OF SUGARCANE

从甘蔗（Ｓａｃｃｈａｒｕｍ ｏｆｆｉｃｉｎａｒｕｍ Ｌ．）嫩叶外植体诱导愈伤组织，经继代培养后，挑选胚性愈伤组织，转入ＭＳ３ 液体培养基，进行悬浮培养。当培养物分离出小粒状的细胞团，细胞变得小而圆时，用于分离原生质体。原生质体以琼脂糖固化的培养方式培养于ＭＲＰ１ 培养基中。由原生质体再生的愈伤组织有两种类型。挑选粒状、坚实的再生愈伤组织转移到Ｎ６ 分化培养基上，“新台糖１ 号”再生的愈伤组织，在含有ＫＴ ０．５ ｍ ｇ／Ｌ的培养基中，分化出绿芽并长成完整的植株。而“粤糖５７－４２３”和“ＵＳ６６－５６－９”再生的愈伤组织，在加有０．１％ 的活性炭的培养基中，前者分化出白化苗。

Calli were induced from yound leaf explant of sugarcane (Saccharum officinarum L.).Through several subcultures embryogenic calli were selected and transfered to liquid MS 3 medium for suspensive culture.When small round cells rich in cytoplasm developed in the suspension cultures, they could be selected for protoplast isolation.Protoplasts were cultured in MRP 1 agarose medium containing sucrose.Protoplast regenerated calli were of two morphological types.Those compact, granular calli were selected for differentation culture on N 6 medium.When placed on N 6 medium containing 0 5 mg/L KT, protoplasts derived calli from ROC 1 regenerated green shoots and eventually grew in to a whole plant.However, only albino plants grew from G.D.57 423 and roots developed from US66 56 9 when they were placed on N 6 medium supplemented with 0 1% activated charcoal with no growth regulator.