摘要
利用钙离子荧光指示剂Ｉｎｄｏ－１ ＡＭ 建立了测定植物细胞胞质游离Ｃａ２＋ 浓度的技术。应用此技术测出，ＢＭＳ（ｂｌａｃｋ Ｍｅｘｉｃｏ ｓｗ ｅａｔ）玉米悬浮细胞原生质体静息水平下的胞质游离Ｃａ２＋ 浓度是１２７±５６ ｎｍ ｏｌ·Ｌ－ １；Ｃａ２＋ 螯合剂ＥＧＴＡ 可使胞质游离Ｃａ２＋ 浓度由７８ ｎｍ ｏｌ·Ｌ－ １降至１２．５ ｎｍ ｏｌ·Ｌ－ １，而Ｃａ２＋ 载体Ａ２３１８７ 则可使胞质游离Ｃａ２＋ 浓度升至接近介质Ｃａ２＋ 水平。同时，证明ＡＢＡ 处理可使ＢＭＳ玉米悬浮细胞原生质体Ｃａ２＋ 浓度在１—１．５ 分钟内迅速升高，由７５ ｎｍ ｏｌ·Ｌ－ １升至７９０ ｎｍ ｏｌ·Ｌ－
The technigue of measuring the concentration of cytoplasmic free calcium ions ([Ca 2+ ]cyt)in plant cell was established using calcium fluorescent indicator Indo 1 AM.The [Ca 2+ ]cyt levels in protoplasts of BMS corn suspension cells under different treatments were measured.It was found that [Ca 2+ ]cyt at resting level was 127±56 nmol·L -1 decreased from 78 nmol·L -1 to 12 5 nmol·L -1 when the cells were treated with calcium chelator (EGTA) and increased nearly to the level of calcium concentration in the medium with the treatment of calcium ionophore A23187.A raid increase of [Ca 2+ ] cyt in protoplasts of BMS corn suspension cells was also demonstrated in 1—1 5 min following ABA treatment.
基金
国家自然科学基金
