摘要
用ＤＮＡ 复性动力学方法克隆到一个水稻中度重复顺序。Ｓｏｕｔｈｅｒｎ 杂交、限制性内切酶分析及序列分析资料表明，该重复顺序在水稻基因组中具有串联重复和散布状态两种存在方式。以该ＤＮＡ 片段作探针，用Ｓｏｕｔｈｅｒｎ 杂交方法分析了多种野生稻种和栽培稻品种的基因组分化特征。某些限制性内切酶消化过的水稻ＤＮＡ，其图谱呈现出多达４０ 条以上的杂交带，包括强杂交带和弱杂交带两种类型。重复实验结果证明，强杂交带表现为ＢＢＣＣ染色体组型特异而弱带则在栽培稻各品种间显示出丰富的多态性。
A moderately repetitive DNA sequence from rice had been cloned by the method of DNA renaturation kinetics.Restriction site analysis, Southern hybridization and DNA sequence analysis indicated that the sequence was mainly in tandem repeat in rice genome.A lot of wild and cultivated rice species were analyzed by Southern blot.With some restriction endonucleases digestion the hybridization pattern of rice DNA showed more than 40 bands with different intensity mainly consisting of the strong and weak hybridization bands.It was found that the strong hybridization bands were specific to the BBCC genome and the weak bands showed abundent polymorphism in cultivated rice species.These results suggested that the repetitive DNA sequence might be useful for rice breeding.
基金
国家863 计划
美国洛氏基金
