摘要
兰州百合(Lilium davidiiDach.)花粉在PEG-400 中萌发,用高度灵敏和特异的t-ZR-IgG和iPA-IgG 免疫亲和层析分离纯化萌发花粉和萌发液中细胞分裂素,并用酶检疫连锁鉴定法(ELISA)测定N6-异戊烯腺嘌呤核苷(t-ZR)和异戊烯基腺苷(iPA)含量。结果表明每克鲜重花粉含有39 ng t-ZR和48 ng iPA。花粉水合后t-ZR 含量略有下降,而iPA 含量明显增高,细胞分裂素总量几乎不变。水合过程中有细胞分裂素的消长,这种变化与花粉管的启动有关。在萌发的前3 小时,花粉管生长速度最快,t-ZR和iPA 在花粉管和萌发液中的含量也随着增加。
Lilium davidii Duch.pollen was germinated in PEG 400.A rapid immunoaffinity chromatograpy system with high efficiency and specificity was adopted to isolate and purify trans zeatin riboside (t ZR) and isopentenyladenosine (ipA).Cytokinin determination was carried out by a highly sensitive enzyme linked immunosolvent assay (ELISA) for t ZR and iPA respectively.t ZR and iPA were present at the level of 10 -8 g/g fr.wt in Lilium davidii pollen.After hydration, t ZR content of pollen decreased slightly, while iPA increased remarkably, and the total amount of t ZR and iPA underwent almost no change.Pollen tubes grew fast in the first 3 h after germination, and the amount of t ZR and iPA also increased greatly both in pollen tubes and in germination medium.The increasing trend of t ZR and iPA and growth rate of pollen tubes was basically synchronous.
基金
国家教委博士点资助
关键词
花粉
细胞分裂素
百合
ELISA
Pollen
Cytokinin
Immunoaffinity chromatography
Enzyme linked immunosolvent assay
Lilium davidii