摘要
研究了大蒜（Ａｌｌｉｕｍ ｓａｔｉｖｕｍ Ｌ．）中期染色体的超微结构和ＲＮＰ物质。常规染色表明，大部分染色体内部有低电子密度区，有的染色体中低电子密度区域较大而似孔洞。银染结果也证明了有大小不等的孔洞存在。Ｂｅｒｎｈａｒｄ 染色显示，在染色体周边和染色体内部都有ＲＮＰ分布。用ＮａＯＨ 处理证明了Ｂｅｒｎｈａｒｄ 染色法所显示的深染区确实含有ＲＮＡ。ＲＮＰ量的多少与ＥＤＴＡ
Chromosomal ultrastructure and ribonucleoproteins (RNP) at mitotic metaphase in garlic (Allium sativum L.) were studied. Areas of low electron is density were observed in the centre of some chromosomes with double staining with Ag staining technic.These areas of low electronic density were further observed as perferated holes. With Bernhard staining technique RNP could be detected in the holes but not evenly distributed. RNA identification was further ascertained by NaOH treatment. The amount of RNP visualized in Bernhard staining technique gradually faded as the duration of EDTA treatment was in creased.
基金
国家教委博士点基金
关键词
染色体
核糖核蛋白
大蒜
Chromosome
Ribonucleoprotein
Allium sativum
