摘要
采用选择性抽提结合 ＤＧＤ（ｄｉｅｔｈｙｌｅｎｅ ｇｌｙｃｏｌ ｄｉｓｔｅａｒａｔｅ） 包埋电镜技术在衣藻（Ｃｈｌａｍｙｄｏｍ ｏｎｓａ ｓｐ．）淀粉核中观察到直径１０ ｎｍ 的纤维网架，以哺乳动物角蛋白抗体进行免疫胶体金标记，在淀粉核区域有很强的特异性结合，间接免疫荧光染色得到相似结果。
The 10 nm diameter filement network in the Chlamydomonas pyrenoid was investigated using selective extraction and diethylene glycol distearate embedding free electron microscopy.The pyrenoid was cross reacted with animal keratin antibody using electron microscopic immunogold labeling.Indirect immunofluorescence observation showed the same result.These suggest that a keratin like intermediate filament network does exist in the Chlamydomonas pyrenoid.
基金
国家自然科学基金
关键词
衣藻
淀粉核
角蛋白
中间纤维网架
Chlamydomonas
Pyrenoid
Intermediate filaments like network
Selective extraction
