外源激素在诱导风信子花被外植体不同部位细胞再生花芽中的作用

ROLE OF EXOGENOUS HORMONES IN INDUCING CELLS IN DIFFERENT POSITIONS OF PERIANTH EXPLANTS TO REGENERATE FLOWER BUDS IN HYACINTHUS ORIENTALIS

外源激素诱导风信子（Ｈｙａｃｉｎｔｈｕｓ ｏｒｉｅｎｔａｌｉｓＬ．）同一发育时期花被外植体不同部位细胞再生花芽的实验表明∶１． 诱导花被外植体细胞再生花芽，外源激素是必需的；２． 仅有细胞分裂素就可以诱导花芽再生，生长素并不是必需的；３． 花被外植体上的不同部位的细胞再生花芽时，需要不同浓度的外源激素． 单独加６－ＢＡＰ或玉米素２ ｍ ｇ／Ｌ可以诱导花被下部的细胞再生花芽；６－ＢＡＰ或玉米素２ ｍ ｇ／Ｌ和２，４－Ｄ ０．１ ｍ ｇ／Ｌ的组合有利于花被中部的细胞再生花芽；６－ＢＡＰ或玉米素２ ｍ ｇ／Ｌ和２，４－Ｄ １．０ ｍ

Role of the exogenous hormone in inducing different position cells of perianth explants of hyacinth to regenerate flower buds was studied.Experiments showed that (1) Exogenous hormones are necessary for inducing cells of the perianth explant to regenerate the flower buds; (2) Only cytokinine alone could induce the regeneration of the flower buds, the auxin was not necessary; (3) Exogenous hormones in different concentrations could induce cells in the different parts of the perianth explants to differentiate the flower buds: 6 BAP or zeatin 2 mg/L alone could induce cells located at the lower part of the perianth to differentiate flower buds.Combination of 6 BAP or zeatin 2 mg/L and 2,4 D 0 1 mg/L was advantageous to cells located middle part of the perianth to regenerate the flower buds.Combination of 6 BAP or zeatin 2 mg/L and 2,4 D 1 0 mg/L could promote cells located at the upper part of the perianth to differentiate flower buds.