油菜素内酯促进绿豆上胚轴伸长与蛋白质和核酸合成的关系

RELATIONSHIP OF THE BRASSINOLIDE PROMOTED EPICO TYL ELONGATION TO PROTEIN AND NUCLEIC ACID SYNTHESIS IN MUNG BEAN CUTTINGS

用饲喂蛋白质和核酸合成的放射性前体［３ Ｈ］－Ｐｈｅ、［３ Ｈ］－尿嘧啶和［３ Ｈ］－胸腺嘧啶证实了油菜素内酯（ＢＲ）能促进绿豆上胚轴的生长和蛋白质、ＲＮＡ 及ＤＮＡ 的合成。用蛋白质和核酸合成抑制剂（ＣＨ、Ａｃｔ．Ｄ、５－Ｆｕ）进一步探讨它们对上胚轴伸长的抑制作用与蛋白质、ＲＮＡ、ＤＮＡ 和ｍ ＲＮＡ 合成之间的关系。证明了上胚轴的伸长依赖于蛋白质和核酸的合成，尤其是依赖于ｍ ＲＮＡ 的合成。说明ＢＲ是在转录水平上调节基因的表达。

Relationship of brossinolide promoted epicotyl elongation to protein, RNA, DNA and mRNA synthesis in mung bean cuttings were studied using radioactive precursors and inhibitors (cycloheximide, actinomycine D and 5 fluorouracil) of protein and nucleic acid synthesis.It was proved that epicotyl elongation of mung bean cutting depended on protein and nucleic acid synthesis, especially mRNA synthesis.This indicates that BR promoted epicotyl elongation was regulated at the level of gene transcription.