摘要
用甲基磺酸乙酯（ＥＭＳ）处理芦苇（Ｐｈｒａｇｍ ｉｔｅｓｃｏｍ ｍ ｕｎｉｓ Ｔｒｉｎ．）胚性愈伤组织。从处理后的愈伤组织诱导获得芦苇耐盐变异植株Ｒ５００２－１２。变异植株能在含有１％ ＮａＣｌ的ＭＳ培养基上生长。细胞学检查变异植株是混倍体，染色体数目变异范围在１００至３３ 之间。
A salt tolerant varaint, R5002 12 of Phragmites communis Trin. has been obtained by treating the embryogenic callis with ethyl methane sulfonate.It could grow in MS medium containing 1% W/V sodium chloride. The plant regenerated was mixoploid with chromosome number ranging from 100 to 33 and its tillers showed similar variation in morphology and chromosome.
基金
国家自然科学基金
关键词
芦苇
甲基磺酸乙酯
耐盐性
变异
细胞学鉴定
Phragmites communis
Salt tolerant variant
Ethyl methane sulfonate
