外源甜蛋白THAUMATINII基因转入烟草的研究

TRANSFER OF SWEET-RASTING PROTEIN THAUMATIN II GENE INTO TOBACCO USING TI-PLASMID VECTOR

利用土壤农杆菌系统，将高甜度的外源甜蛋白ｔｈａｕｍａｔｉｎＩ１基因转入烟草细胞，并得到大量转基因植株及其后代。经分子杂交分析确证ｔｈａｎｍａｔｉｎＩＩ基因已整会到烟草植株的基因组中，并在转录水平检测到表达。标记基因胭脂碱合成酶（ＮＯＳ）基因及新霉素磷酸转移酶（ＮＰＴＩＩ）基因也在转基因植株中正常表达。

The gene for intensely Sweet-tasting Protein thaumatin Il with 35S promotor of CaMV was transferred into leaf disks of cultivar Nicotiana tabacum L,G28 with the belp of Ti-plasmid of Agrobacterium tumefaciens,These leaf disks were co-cultivated wit A,tumefaciens harbo- ring a co-integrated vector with a thaumatin II gene, a neomychphosphotransferase(NPT II)gene and a nopaline synthase(NOS)gene,Many of transgenic plants were regenerated and rooted on medium containing kanamycin, Most of tha transgenic plants exhibited both NPT Il and NOS enzyme activities,Results of Sonthern blot and Northern blot showed that thaumatin II gene was stably integrated and transcripted in transgenic plants。