摘要
一定浓度的盐或山梨醇胁迫可促使粉状毕赤酵母(Pichiafarinosa)过量表达3 磷酸甘油脱氢酶(Glyc erol 3 Phosphatedehydrogenase) ,从而合成出大量甘油抵御胁迫。研究中发现,在5 %盐胁迫下,粉状毕赤酵母粗酶液3 磷酸甘油脱氢酶酶活提高30 % ,在6 %盐胁迫下,甘油产量提高了9倍。在1 5mol/L的山梨醇胁迫下,3 磷酸甘油脱氢酶酶活提高了14 %。
Pichia farinose would express superfluous Glycerol-3-Phosphate dehydrogenase and synthesize lots of glycerol against stress in salt and D.sorbitol. We have found that activity of Glycerol-3-Phosphate dehydrogenase in Pichia farinose in 5% salt is 30% higher than that with no salt and glycerol-output of Pichia farinose in 6% salt is 9 time higher than control. Activity of Glycerol-3-Phosphate dehydrogenase in 1.5mol/L D.sorbitol is 14% higher than without adding D.sorbitol.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2005年第3期5-7,共3页
Food and Fermentation Industries
基金
国家高技术发展计划 (863计划 ) (No .2 0 0 2 .AA2 1 2 0 2 1 )
